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biotinylated puromycin  (MedChemExpress)


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    Structured Review

    MedChemExpress biotinylated puromycin
    Biotinylated Puromycin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+puromycin/Puromycin-bis(PEG2-amide)-Biotin/bio_rxiv__64898__2026__02__21__707190-404-17-21
    Average 94 stars, based on 1 article reviews
    biotinylated puromycin - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: eIF4E and Ezrin cooperate in pseudopods to drive a localized migratory translation program in acute myeloid leukemia
    Article Snippet: Cells were then washed three times with RL buffer and blocked for 30 minutes at RT with 2% BSA with 2mg/ml Biotin (cat# B1595, Invitrogen) in RL buffer. .. After washing three times with RL buffer, cells were incubated for 1 hour at RT with 50mM Biotinylated Puromycin (cat# 2379889-82-2, MedChemExpress) in RL buffer with 2% BSA. .. After washing three times with RL buffer, cells were incubated with 2mg/ml NeutrAvidin Oregon Green 488 (cat# A6374, Invitrogen) in 2% BSA in RL buffer for 1 hour at RT, washed three times with RL buffer and either mounted in ProLong TM gold antifade media with DAPI, or stained for proteins as indicated in the text.



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